User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/24: Difference between revisions
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* Not all membranes were activated again, while they were maintained under moist conditions | * Not all membranes were activated again, while they were maintained under moist conditions | ||
* Column had fallen dry and cracked, this was not noticed until after addition of sample. Separation appeared still succesful | * Column had fallen dry and cracked, this was not noticed until after addition of sample. Separation appeared still succesful | ||
==Results== | ==Results== | ||
*Rabbit Pre-immune Western blot | *Rabbit Pre-immune Western blot | ||
[[Image:24082010_WB_Rabbit_Pre-immune_SERUM.tif|500px]] | [[Image:24082010_WB_Rabbit_Pre-immune_SERUM.tif|500px]] | ||
==Discussion== | ==Discussion== | ||
* | * Pre-immune was not only IgG but whole serum this should explain the high amount of signals seen. Dörte is indisposed at the moment so I am unable to ask here where pre-immune IgG is to be found. | ||
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==Related== | ==Related== |
Revision as of 08:49, 24 August 2010
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RII Overlay, Pre-IgG 2nd AB, CSE on HEK293, ASMC to S0, Split cells w. Mileh | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Materials & MethodsMaterials
MethodWestern blot Rabbit pre-immune IgG
RII Overlay
CSE stimulation HEK293
Put ASMC to S0
NotesRII overlay
Results
Discussion
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