840:153g:Projects/project4/2009/04/15

MoldBusters' Journal

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Wednesday 4/15 Josh The controls for the transformation showed that the transformation worked properly, and that ampicillin resistance was being selected for. For the ligation controls, only two colonies grew which contained only the plasmid. The most efficient ligation was the 1:1 ratio, and plated in an 8μL quantity. This plate showed 50 colonies. Other colonies were observed on the 2:1 ratio. Eleven colonies from the 1:1 ligation and one from the 2:1 ligation were used to inoculate liquid cultures containing ampicillin. Incubated at 37°C , to be used tomorrow for plasmid extraction.