840:153g:Projects/project4/2009/04/15

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Wednesday 4/15

Josh

  • The controls for the transformation showed that the transformation worked properly, and that ampicillin resistance was being selected for.
  • For the ligation controls, only two colonies grew which contained only the plasmid.
  • The most efficient ligation was the 1:1 ratio, and plated in an 8μL quantity. This plate showed 50 colonies. Other colonies were observed on the 2:1 ratio.
  • Eleven colonies from the 1:1 ligation and one from the 2:1 ligation were used to inoculate liquid cultures containing ampicillin. Incubated at 37°C , to be used tomorrow for plasmid extraction.


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