IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/30

iGEM Project name 1

Main project page Previous entry      Next entry

Miniprep of Lock, Key, and Control Lock constructs from July 28 performed using Raf's Alkaline Lysis protocol Miniprepped DNA was diluted to 100μg/μL and sent for sequencing Results of M9 Minimal Media Test After 16 hours, the only cultures that showed any growth were the cultures in the LB-chlor+1% arabinose. The cultures started in the M9 minimal media did not show any change in OD Further examination of the BW27783 strain shows that they are not able to metabolize arabinose, this would explain the lack of growth in the Minimal Media 4mL of 100% glycerol was added to each of the 3 batches of M9 Minimal Media as a carbon source, creating a final concentration of 0.4%. As glycerol is a less preferred sugar then arabinose, it should allow the cells to grow while preferentially importing arabinose into the cell Overnights with M9 Minimal Media To test if the cells will grow in gycerol-arabinose M9 minimal media Started J23100, pBAD(wt)+GFP, pBADweak+GFP and J23101 in 3mL each of M9 Minimal Media batches B and C.