IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct/Design
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Super Parts | Prey Construct | ||
---|---|---|---|
Actual Part | T9002: Test Sensing Prey Construct | ||
Sub Parts | F2620 | intermediate_part | intermediate_part |
Registry
- <bbpart>BBa_T9002</bbpart>
Design choices
- This part is already available in the Registry
- It has the following properties that we need in order to characterize the Prey Sensing Part:
- The part responds to AHL levels, via activation of pLuxR
- From this, we can calibrate a AHL assay based on fluorescence
- LuxR is under the control of pTet, allowing different levels of LuxR to be achieved
- AHL Levels:
- The model is sensitive to initial AHL levels
- As AHL input increase, GFP output increases until saturation
- The relationship between AHL level and GFP output is described by the equation above
- The exact relationship can be calculated from this assay
INPUTS | biological component | comments |
---|---|---|
Prey molecule | AHL | Homoserine lactone synthesized by the LuxI gene <bbpart>BBa_C0061</bbpart> |
OUTPUTS | biological component(s) | comments |
Pops | GFP | Green Fluorescent Protein <bbpart>BBa_E0040</bbpart> |
Open issues
A potential issue with the experiment is regarding using pTet as the promoter for LuxR expression. It may be necessary to add a gene for TetR expression to ensure control over this promoter.