User:Klare Lazor/Notebook/Chem-496-001/2012/03/27

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab Main project page
Previous entry      Next entry


Description

  • TODAY DIALYSIS OF GOLD NANOPARTICLES IN DIFFERENT VOLUMES
  • ADD BSA CONCENTRATION TO BSA CONCENTRATION CURVE

Dialysis

  • Membrane
    • Spectra/PorBiotech Cellulose Ester (CE) Dialysis Membrane MWCO: 100,000
    • 100 kD
    • wet in 0.05% sodium azide
  • 1
    • about 1,000 ml water bath
    • 5 ml AuNPs b1
  • 2
    • 100 ml water bath
    • 3.5ml AUNP b2

Data

Image:AUNPs_before_dialysis_batch_1_and_2.png

Figure 1: image of absorbance of AuNPs before dialysis for batch 1 and batch 2, after dialysis uv spectra will be taken again to obtain the concentration of bsa bound to the nano particles.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


Personal tools