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| [[Category:Protocol]]
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| Protocol for passing cells on fibronectin-coated dishes.
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| 1. Prepare fibronectin-coated dishes.
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| Put 150ul fibronectin solution (20-40 ug/ml in water) at the center of glass-bottom dish, then overnight in CO2 incubator or 2 hours without cover in hood at RT.
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| 2. Detach cells (HeLa, MEF) with 4 mM EDTA(in PBS) in CO2 incubator for 15 min, then slightly blow down the cells from the bottom of culture dish. The other steps are similar as normal pass.
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| 3. Usually before imaging, incubate cells on fibronectin-coated dishes in CO2 incubator for 1-6 h.
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| ==''In vivo''== | | ==''In vivo''== |
