Maheshri:Internal: Difference between revisions
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*[[Maheshri:RTPCR| RT-PCR]] | *[[Maheshri:RTPCR| RT-PCR]] | ||
*[[Maheshri:FluorescenceM | Fluorescence Microscopy]] | *[[Maheshri:FluorescenceM | Fluorescence Microscopy]] | ||
*[[Maheshri:3C | Cromatin Conformation Capture (3C)]] | |||
=Software= | =Software= | ||
Revision as of 12:48, 27 March 2012
General Information
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Standard Operating Procedures for the Protocol
- Common Area Etiquette
- Locations of Common Items and Reagents
- Enzymes
- Abbreviations
- Equipment
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- Sporulation in Liquid Media
- PCR
- Kinase and Anneal Oligos
- Restriction Digestion
- Agarose gel electrophoresis
- Gel Extraction
- DNA Ligation (OpenWetWare)
- Making Chemically Competent Bacteria
- Making Electro-competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Transformation by Electroporation
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
- Cromatin Conformation Capture (3C)
Software
- MATLAB
- VectorNTI; speak to the Computer Guru about obtaining a copy. VectorNTI tutorials & reference guide
- Cell tracking and analysis for time-lapse fluorescent microscopy
