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<img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People">
</html>The Team
The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors.
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Questions to ask the fluorometer Engineer on Friday, Aug 3
See if our plate reader has the ability to measure what you want to measure.
What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
Temperature-regulation?
How do we subtract background values from data and convert from absorbance to OD600?
Talk about using the plate reader and creating protocols.
Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
Any manufacturer protocols for plate reader usage?
Well plate rows and columns? How is data arranged sequentially with the wells?
What kind of plates you need to order and use for our experiments?
Lamp energy and Noise signal ratios??
Talk about processing data
Export format?
How to export data from fluorometer?
Built-in data analysis tools?
Technical Support.
Experiment 1: Fluorescence vs extracellular [GFP] molecules
Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.
Materials Required
Equipment
Reagents
Protocol
Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]
Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].
Materials Required
Equipment
Reagents
Protocol
Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]
Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].
