User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> UV-vis of AuNP and Tris Over Time</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effect of Tris Buffer on Protein Fibers III</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
==Objective==


 
To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]].


==Description==
==Description==


# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]].
# Centrifuge AuNP fibers + 50 mM tris solution made on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] at 13200 rpm for 10 min.
# Take UV-vis absorption data.
# Take UV-vis absorption data.
# Compare data to that obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] and [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21 |02/21/12]].
# Compare data to that obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]] and [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21 |02/21/12]].

Revision as of 10:39, 7 March 2012

Effect of Tris Buffer on Protein Fibers III <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12.

Description

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12 at 13200 rpm for 10 min.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12 and 02/21/12.

Data

  • Graph of absorbance vs wavelength over time for the AuNP fibers + 50 mM tris after centrifuge:

Overall absorbance decreased this week, however it appears that the peak wavelength has continued to shift lightly towards shorter wavelengths.

Notes



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