User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/29: Difference between revisions
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=Objectives= | =Objectives= | ||
* Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]. | * Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] and liquid for extraction. | ||
* See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| | * See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| extractions]] of pUX19 cut with SmaI. | ||
* Digestion of pSB1AK3/BBa_B0015 with XbaI to see size. | |||
==Gel of SmaI restriction== | ==Gel of SmaI restriction== | ||
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Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction. | Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction. | ||
==Extraction== | |||
Extraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep. | |||
[[Image:PartBBa B0015.KAres.29jul2011.JPG|120px]] | |||
I just need it to see concentration for digestion. | |||
==Digestion== | |||
{| border="1" | |||
!Reactives | |||
!μL | |||
|-- | |||
|XbaI | |||
|1 | |||
|-- | |||
|Buffer4 NEB10X | |||
|2 | |||
|-- | |||
|BSA10X | |||
|2 | |||
|-- | |||
|DNA of pSB1AK3/BBa_B0015 | |||
|12 | |||
|-- | |||
|H<sub>2</sub>O | |||
|3 | |||
|-- | |||
|Total | |||
|20 | |||
|} | |||
Over night reaction. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 00:11, 2 August 2011
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Objectives
Gel of SmaI restrictionI expect 6.2Kbp~
Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction. ExtractionExtraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep. I just need it to see concentration for digestion. Digestion
Over night reaction.
|