Berglund:Protocols
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http://openwetware.org/wiki/Berglund_Lab
Contents
1
Gels
2
Nucleic Acid
3
Structure
4
Protein
5
Cloning and Bacteria
6
General
7
Antibody Work
8
Splicing
9
Nanoparticle Work
Gels
Agarose
SDS-PAGE
Acrylamide
Native Acrylamide
Acrylamide-Agarose
Nucleic Acid
PCR: Commonly Used Primers
PCR: Various Methods
Transcription: Various Methods
Kinasing
Phenol/Ethanol Extraction
RNA column purification
making rNTP
Structure
Footprinting
UV crosslinking
SHAPE
Crystallography
Analytical computer programs
Protein
Protein Buffers
Protein Purification: Various Methods
Binding Gels: Various Methods
Protein Concentration Assay: Various Methods
Western Blot
Coomassie Blue Staining
Silverstaining
Cloning and Bacteria
Cells Available
Making Competent Cells: Various Methods
Chemical Transformation
Electroporation
Cloning: Various Methods
General
Buffers
Machines Available for Use
Formerly Used Protocols
Worm Fix
Antibody Work
List of Antibodies Available
Antibody Storage
Antibody Purification
Antibody Making/Ordering
Splicing
In Vivo
Cells Available
General Cell Culture Methods
Solutions
Methods of Analysis
In Vitro
Background
Complex Formation
Splicing
Nanoparticle Work
Reagents
Method A
Method B
Using the TEM
Equipment available
Category
:
Berglund Protocols
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