Protocols: Difference between revisions
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[[Category:Protocol]] | |||
Protocol for passing cells on fibronectin-coated dishes. | |||
1. Prepare fibronectin-coated dishes. | |||
Put 150ul fibronectin solution (20-40 ug/ml in water) at the center of glass-bottom dish, then overnight in CO2 incubator or 2 hours without cover in hood at RT. | |||
2. Detach cells (HeLa, MEF) with 4 mM EDTA(in PBS) in CO2 incubator for 15 min, then slightly blow down the cells from the bottom of culture dish. The other steps are similar as normal pass. | |||
3. Usually before imaging, incubate cells on fibronectin-coated dishes in CO2 incubator for 1-6 h. | |||
==''In vivo''== | ==''In vivo''== |
Revision as of 13:02, 17 June 2007
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