WangLab:Protocols

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Cell Biology

Protocols Biotin Avdin Blocking Coat Beads with Fibronectin c-Src Mutations Immunoprecipitation and Immunoblotting Immunostaining Extramembrane Signals Immunostaining General Coupling Protein Carboxyl Beads Cell Culture Protocols Clustering of Epherin Covalently Coupling Beads EGF Experiment Immunostaining Biotin PH Domain GFPs PIPs Demirovic Proteasephosphatase Purify Protein Invitro Kinase Assay Ras and Src Treatment Protocol RGD Peptide Inhibitions Simple Purification of Adenovirus Staining Biotin Stimulation of Integrins Transformation Procedure Lipofectimine 2000

Materials Antibodies Cell Culture Dishes Cell Culture Mediums HEPES buffer Inhibitors Integrin Antibodies Lysis Buffer Pervanadate

Molecular Biology

Protocols Competent Cell Preparation Deletion and Addition Inserts Deletion or Insertion of Amino Acids w/o the Involvement of RE sites DNA Digestion FRET Related Techniques Hamster c-ha-ras_cds Ligation of Reporter to Adenovirus Ad5 Ligation Samples Membrane Binding Domain Review Multiple Mutations PCR Programs PCR Reaction Protocol Primer Design Standard PCR

Materials

Nanotechnology

Standard Protocol to Fabricate Silicon Master

Protocols for Coupling Carboxyl Beads to EGF or Fibronectin

Optics and Bio-Photonics

Protocols Alignment of Microscopy Conversions Confocal Microscopy Usage DIC Microscope Setting Rules to Follow for Microscopy FRAP Experiment Protocol

Materials