Maloof Lab:Protocols

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[[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]]
[[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]]
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[[Maloof_Lab:Ultra-competent E.coli|<font style="color:#000;">Making ultra-competent E.coli (Inoue method)</font>]]
[[Maloof_Lab:Cell_Transformation|<font style="color:#000;">E. coli (DH5α chemically-competent) transformation</font>]]
[[Maloof_Lab:Cell_Transformation|<font style="color:#000;">E. coli (DH5α chemically-competent) transformation</font>]]
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[[Maloof_Lab:MS_plates|<font style="color:#000;">MS plates for plant growth</font>]]
[[Maloof_Lab:MS_plates|<font style="color:#000;">MS plates for plant growth</font>]]
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[[Maloof_Lab:MS_plates2|<font style="color:#000;">MS plates for plant growth (buffered, no sucrose)</font>]]
[[Maloof_Lab:Exo_SAP|<font style="color:#000;">Exo-SAP PCR cleanup for sequencing</font>]]
[[Maloof_Lab:Exo_SAP|<font style="color:#000;">Exo-SAP PCR cleanup for sequencing</font>]]
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[[Maloof_Lab:Seed_Storage|<font style="color:#000;">Seed Storage</font>]]
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[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>]
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[[Maloof_Lab:Trizol|<font style="color:#000;">Trizol extraction</font>]]
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<h3><font style="color:#C9D3EB;">Stocks</font></h3>
<h3><font style="color:#C9D3EB;">Stocks</font></h3>
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[[Maloof_Lab:PCR_stuff|<font style="color:#000;">PCR taq, buffer and MgCl<sub>2</sub></font>]]
 
[[Maloof_Lab:antibiotics|<font style="color:#000;">Antibiotics</font>]]
[[Maloof_Lab:antibiotics|<font style="color:#000;">Antibiotics</font>]]
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[[Maloof_Lab:buffers_electrophoresis|<font style="color:#000;">Electrophoresis Buffers</font>]]
[[Maloof_Lab:buffers_electrophoresis|<font style="color:#000;">Electrophoresis Buffers</font>]]
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[[Maloof_Lab:loading_dye|<font style="color:#000;">Loading Dye</font>]]
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[[Maloof_Lab:loading_dye|<font style="color:#000;">Loading Dye and 1kb Ladder</font>]]
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[[Maloof_Lab:PCR_stuff|<font style="color:#000;">PCR taq, buffer and MgCl<sub>2</sub></font>]]
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<h3><font style="color:lightblue;">Lab Tasks</font></h3>
<h3><font style="color:lightblue;">Lab Tasks</font></h3>
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[[Maloof_Lab:Lab_Tasks|Maloof lab tasks]]
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[[Maloof_Lab:Lab_Tasks|<font style="color:#000;">Maloof lab tasks</font>]]
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[[Maloof_Lab:Undergrad_Instructions|<font style="color:#000;">Undergrad Instructions</font>]]
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<h3><font style="color:#F8B603;">Scripts, instructions and miscellaneous</font></h3>
<h3><font style="color:#F8B603;">Scripts, instructions and miscellaneous</font></h3>
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[[Maloof_Lab:Epson_Scanner|<font style="color:#000;">Using the Epson scanner</font>]]
[[Maloof_Lab:LI1800_light_meter|<font style="color:#000;">Using the LI1800 light meter</font>]]
[[Maloof_Lab:LI1800_light_meter|<font style="color:#000;">Using the LI1800 light meter</font>]]
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[[Media:Black_Comet_V1.doc‎|<font style="color:#000;">Using the Black Comet light meter</font>]]
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[[Maloof_Lab:eBioX|<font style="color:#000;">Sequence Analysis with eBioX</font>]]
[[Maloof_Lab:ImageJ_analysis|<font style="color:#000;">Analyzing luminiscence over time with ImageJ</font>]]
[[Maloof_Lab:ImageJ_analysis|<font style="color:#000;">Analyzing luminiscence over time with ImageJ</font>]]
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[[Maloof_Lab:Hypocotyl_measurement|<font style="color:#000;">Hypocotyl measurement with ImageJ</font>]]
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[http://ostrander.ucdavis.edu/~maloofweb/cgi-bin/ <font style="color:#000;">Maloof/Harmer Primer Database Search</font>] (Only internal users)
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[http://ostrander.ucdavis.edu/~maloofweb/cgi-bin/Patricia <font style="color:#000;">Patricia's Motif search </font>] (Only internal users)
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[http://whitney.plb.ucdavis.edu/gene_exp_plot/form.html <font style="color:#000;">Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN</font>] (Only internal users)
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[http://whitney.plb.ucdavis.edu/gene_exp_4_species/form.html <font style="color:#000;">Maloof Gene Expression Plot Tool for M82, PEN, HAB  & PIM</font>] (Only internal users)
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[[Maloof_Lab:Laser_tough_tags_settings|<font style="color:#000;">Laser Tough Tag Settings to print</font>]]
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[[Maloof_Lab:How_to_print_barcodes|<font style="color:#000;">How to print barcodes</font>]]
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[[Maloof_Lab:PDA_bluetooth_calipers|<font style="color:#000;">PDA measurement system</font>]]
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[[Maloof_Lab:ImageJ_Time_Lapse_Hypocotyls|<font style="color:#000;">Measuring time-lapse hypocotyl movies with ImageJ</font>]]
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Revision as of 15:16, 17 December 2012

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Protocols

DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

Making ultra-competent E.coli (Inoue method)

E. coli (DH5α chemically-competent) transformation

Agrobacterium transformation (Freeze-Thaw method)

Cleaning ball bearings

Seed sterilization

Miniprep using Promega reagents but not the columns

MS plates for plant growth

MS plates for plant growth (buffered, no sucrose)

Exo-SAP PCR cleanup for sequencing

Seed Storage

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

Trizol extraction

Stocks

Antibiotics

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Maloof lab safety

Lab Tasks

Maloof lab tasks

Undergrad Instructions

Scripts, instructions and miscellaneous

Using the Epson scanner

Using the LI1800 light meter

Using the Black Comet light meter

Sequence Analysis with eBioX

Analyzing luminiscence over time with ImageJ

Hypocotyl measurement with ImageJ

Maloof/Harmer Primer Database Search (Only internal users)

Patricia's Motif search (Only internal users)

Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN (Only internal users)

Maloof Gene Expression Plot Tool for M82, PEN, HAB & PIM (Only internal users)

Laser Tough Tag Settings to print

How to print barcodes

PDA measurement system

Measuring time-lapse hypocotyl movies with ImageJ


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