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- 08:19, 2 May 2024 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Buffer HEPES (Created page with "'''Buffer HEPES: 500 mL, pH 7.4 ''' '''Este buffer no puede ser usado para trabajar con Calceina, en ese caso es obligatorio usar un buffer de elución.''' ==Materiales== * HEPES 2,383 g * NaCl 3,1896 g * NaOH 1 M * HCl 1 M ==Procedimiento== # Colocar 400 ml de agua deionizada en la botella con agitador. # Diluir el HEPES y el NaCl # Ajustar pH a 7.4 usando NaOH y/o HCl. # Sacar el agitador y completar los 500 mL con agua deionizada {| class="wikitable" style="...")
- 07:53, 2 May 2024 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Elution Buffer8 mOsM ESP (Created page with " ==Materiales== * HEPES 9.532 g * NaCl * NaOH 1 M 30 ml * HCl 1 M ==Procedimiento== # Colocar 1.8 litros de agua deionizada en la botella con un agitador. # Diluir el HEPES (concentracion final de 20 mM) y el NaCl (concnetracion final de 170 mM). # Ajustar pH a 7.4 usando NaOH y/o HCl (Generalmente se inicia con pH de 5.4) # Determinar la concentración de HCl y NaOH. Por ejemplo, si entre los dos se agregaron 16 ml a 1M, entonces para un buffer de 2 L la concentrac...")
- 08:30, 29 April 2024 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Calcein8 ESP (Created page with "'''Calceina: 800 mOsM, 50 mM, 50 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * NaCl * 1.5564 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 10 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 1.5564 g de calceina (50 mM o 50 mOsM) # Agregar 200 mM de NaCl (...")
- 07:39, 2 April 2024 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Elution Buffer ESP (Created page with " ==Procedimiento== # Colocar 1.6 litros de agua deionizada en la botella con un agitador. # Diluir el HEPES (concentracion final de 20 mM) y el NaCl (concnetracion final de 170 mM). # Ajustar pH a 7.4 usando NaOH y/o HCl. # Determinar la concentración de HCl y NaOH. Por ejemplo, si entre los dos se agregaron 16 ml a 1M, entonces para un buffer de 2 L la concentración es de 8 mM. # Calcular la osmolaridad y completar los 400 mOsm con NaCl. # Sacar el agitador y co...")
- 14:34, 14 February 2024 Elizabeth Suesca talk contribs created page UA Biophysics: Extruder Cleaning Instructons ESP (Created page with "Tenga en cuenta que nunca se deben sonicar los émbolos. #Enjuagar con abundante agua los tambores, jeringas y el cilindro metálico #Sonicar con agua desionizada los tambores (10 minutos), si nota que el agua sale muy verde, cambie el agua y sonique nuevamente. #Sonicar con etanol 95% los tambores (10 minutos), #Purgar 10 veces las jeringas conectadas a los tambores con agua desionizada y 10 veces más con alcohol al 95%. #En caso de que no se logre limpiar por comple...")
- 14:08, 10 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Lyophilizer ESP (Created page with " thumb|right|240px '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>ANTES DE ENCENDER:</h2> #Revise que la cámara del liofilizador este alineada y seca. #Si no lo está, límpiela muy bien usando una toal...")
- 08:23, 9 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Plasma Cleaner ESP (Created page with " thumb|right|240px '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' ''' Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>FUNCIONAMIENTO CON AIRE:</h2> #Abra la válvula de la bomba de vacío y cierre la válvula de precisión. #La flecha en la válvula p...")
- 09:50, 8 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Spectrofluorometer Instructions ESP (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo.''' <h2>TURN ON THE EQUIPMENT: </h2> #Turn off computer and instrument before starting the lamp power supply. #Set the current knob of the lamp power supply completely counter-clock-wise turn th...")
- 09:45, 8 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Autoclave Instructions ESP (Created page with " '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>ANTES DE ESTERILIZAR:</h2> # El material debe estar limpio. # Los objetos metálicos y pipetas deben estar envueltos en papel. # Coloque la cinta indicadora en cada frasco y dentro de...")
- 13:15, 26 June 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Lipid Extration ESP (Created page with "==Materials for 1 g of Cell== * Células SA401 pesadas y maceradas con mortero * Cloroformo * Metanol Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * Perlas de vidrio * Solución de NaCl 1.7 M (10% W/V) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Fascos de vidrio de 50 ml '''Nota:''' Preparar una mezcla de cloroformo:metanol (2:1) (20 ml de cloroformo/10 m...")
- 09:04, 1 June 2023 Elizabeth Suesca talk contribs created page File:Cloroformo.png
- 09:04, 1 June 2023 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png
- 08:25, 1 June 2023 Elizabeth Suesca talk contribs created page File:Acetato.png
- 08:25, 1 June 2023 Elizabeth Suesca talk contribs uploaded File:Acetato.png
- 14:39, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Carotenoid Extration ESP (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")
- 14:07, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Elution Buffer (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")
- 14:00, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Calcein ESP (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")
- 08:31, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")
- 12:57, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Bacteria for Lipid Extraction (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")
- 12:17, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Erythromycin (Created page with "Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains. ==STOCK SOLUTION== Erythromycin is used in the biophysics lab at a 2 ug/ml. Stock solutions are at 1 mg/mL ==PROTOCOL (STORAGE)== According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 20mL of 25mg...")
- 11:58, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Calcein (Created page with "Receta para 100 ml, 50mM, 400 mOsM, pH 7,4 Materiales: Probeta 100 ml 30 ml de NaOH 3,113 g de calceina Procedimiento: En probeta 20 ml de NaOH 1M (colocque 21 ml) Disolver en el NaOH 3,113 g de calceina Mezclar 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) y HEPES 238.3 mg (para que quede a 10 mM).completar un volumen de 40 ml Agregar la mezcla a la calceina Ajustar pH con HCl 1M (2 ml) Entre HCl y NaOH se agregaron 2...")
- 06:39, 20 January 2023 Elizabeth Suesca talk contribs created page File:Desionizador Partes.jpg
- 06:39, 20 January 2023 Elizabeth Suesca talk contribs uploaded File:Desionizador Partes.jpg
- 06:38, 20 January 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Water Purification System (Created page with " '''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #Remove the cap and place your flask. #Press “DISP” #Press “DISP” (RO, destilled) o “FLUSH” (UP, deionized) to choose the water quality. Ensure that the pure water at lea...")
- 07:53, 19 January 2023 Elizabeth Suesca talk contribs created page File:Desionizador.jpg
- 07:53, 19 January 2023 Elizabeth Suesca talk contribs uploaded File:Desionizador.jpg
- 07:48, 19 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Incubadora refrig.jpg
- 07:45, 19 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Incubadora.jpg
- 08:11, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Syringe.jpg
- 08:10, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Plasma.jpg
- 08:09, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Spectrofluorimeter.jpg
- 08:05, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Horno.jpg
- 07:42, 18 January 2023 Elizabeth Suesca talk contribs created page File:Espectrofotometro Partes.jpg
- 07:42, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:Espectrofotometro Partes.jpg
- 07:34, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Hot.jpg
- 07:33, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Centrifuga.jpg
- 07:29, 18 January 2023 Elizabeth Suesca talk contribs created page File:UA NanoDrop.JPG
- 07:29, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:UA NanoDrop.JPG
- 07:27, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Sonicador.jpg
- 07:21, 18 January 2023 Elizabeth Suesca talk contribs created page File:Sonicador-partes.jpg
- 07:21, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:Sonicador-partes.jpg
- 07:15, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Logichepamain.jpg
- 07:12, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Balanza-analitica-xb-220a-precisa.jpg
- 07:05, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Liofilizador.jpg
- 06:56, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Autoclave.jpg
- 14:18, 17 January 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Spectrophotometer (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #The cell holder have to be empty before turning on the instrument. #Turn on the instrument using the power switch on the back panel. #Wait almost 20 min before using it. Spectrometer...")
- 08:28, 22 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Centrifuge Primo R (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Quick Start</h2> #Turn on the mains switch on the back of the instrument. #After internal check, the display switches to the actual values. #Set temperature, velocity, and time using the UP/DOWN KEY...")
- 07:26, 17 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Syringe Pump (Created page with "[https://www.harvardapparatus.com/media/manuals/Product%20Manuals/11%20Elite%20Manual_5420-002REV1.0.pdf Operator´s Manual] Return to equipment")
- 06:30, 17 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: NanoDrop (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Quick Start</h2> #Double-click the software icon and select the software application of interest from the right pane. Select “Add to report” prior to a measurement to save the sample data to a wor...")
- 12:26, 16 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Sonicador (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Functions</h2> '''MAIN POWER SWICH:''' This switch is located on the back of the unit, next to the power cord receptacle. '''ON/STANDBY:''' When the Main Power switch on the rear panel is in the ON...")