WangLab:Protocols

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Cell Biology

Protocols Biotin Avdin Blocking Cell Culture Protocols Cell Imaging General Protocol (MEF) Clustering of Epherin Coat Beads with Fibronectin Coupling Protein Carboxyl Beads Covalently Coupling Beads c-Src Mutations EGF Experiment Immunoprecipitation and Immunoblotting Immunostaining Extramembrane Signals Immunostaining General Immunostaining Biotin Lipofectimine 2000 PH Domain GFPs PIPs Demirovic Proteasephosphatase Purify Protein Invitro Kinase Assay RGD Peptide Inhibitions Ras and Src Treatment Protocol Simple Purification of Adenovirus Staining Biotin Stimulation of Integrins Transformation Procedure

Materials Antibodies Cell Culture Dishes Cell Culture Mediums Coat Glass Bottom Dish with Fibronectin HEPES buffer Inhibitors Integrin Antibodies Lysis Buffer Pervanadate Fabrication of glass bottom dishes

Molecular Biology

Protocols Competent Cell Preparation Deletion and Addition Inserts Deletion or Insertion of Amino Acids w/o the Involvement of RE sites DNA Amplification (Mini prep) DNA Amplification (Maxi prep) DNA Digestion Electro-competent FRET Related Techniques Hamster c-ha-ras_cds Ligation of Reporter to Adenovirus Ad5 Ligation Samples Maxiprep procedure Measure DNA Concentration Membrane Binding Domain Review Multiple Mutations PCR Programs PCR Reaction Protocol Primer Design Standard PCR

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Nanotechnology

Protocols Protocols for Coupling Carboxyl Beads to EGF or Fibronectin PDMS Training Standard Protocol to Fabricate Silicon Master

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Optics and Bio-Photonics

Protocols Alignment of Microscopy Lab Microscope Parameters Conversions Confocal Microscopy Usage DIC Microscope Setting FRAP Experiment Protocol Rules to Follow for Microscopy

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Manuals

Protocols Operation of Vacuum Oven

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